Journal: Cancers

Article Title: Tumor Cells and the Extracellular Matrix Dictate the Pro-Tumoral Profile of Macrophages in CRC

doi: 10.3390/cancers13205199

Figure Lengend Snippet: The impact of MHC-II expression on the antigen presentation ability of monocyte-differentiated macrophages to T lymphocytes. PBMCs were isolated from tetanus-vaccinated subjects and stimulated with 15% CCD841 (IEC) conditioned medium and 15% HCT-116 (CRC) conditioned medium (left panel) or stimulated with 25% co-culture medium of monocytes + the HC D-ECM and the monocytes CRC D-ECM (right panel) in the presence of 0.5 µg/mL of tetanus toxoid. Lymphocyte proliferation was measured by 3 [H]-TdR incorporation. Data are expressed as n -fold vs. IEC or vs. the normal D-ECM ± SEM of triplicate samples from two different subjects. Significance was determined by Student’s t -test: ** p < 0.01 and **** p < 0.0001.

Article Snippet: Normal human intestinal cells CCD841 CoN (ATCC ® CRL-1790TM) were cultured in EMEM 10% FBS, 10 mM Hepes, 100 U/mL of penicillin, and 100 μg/mL of streptomycin.

Techniques: Expressing, Immunopeptidomics, Isolation, Co-Culture Assay